教員個人(研究室)ページ
植物適応制御学研究室
Laboratory of Plant Environmental Adaptation
＜研究略歴＞
2006年、東京理科大学基礎工学研究科生物工学専攻博士課程修了。ベルギー・VIB/ゲント大学にて博士研究員、理化学研究所植物科学研究センターにて特別研究員、奈良先端科学技術大学院大学にて博士研究員、助教を経て、2023年より現職。専門は植物科学。＜主な担当科目＞
植物生命科学、植物工学、生命科学実験＜ひとこと＞
諦めずに挑戦し続けることが、大きな発見につながる研究テーマ
自由に移動できる動物とは異なり、陸上植物は一度根付いた場所から移動することはできません。そのため、周りの環境からの高温や乾燥、紫外線などの様々なストレスに対して、柔軟に対処する必要があります。そして、植物はそれらストレスに適応するために、自らの成長を周りの環境に合わせて適切にコントロールしています。
私たちは、植物がストレスを受けた時に、どのようにそれらストレスを認識し、そのストレスに対してどのように応答することで、ストレス環境下での成長、生存を可能にしているのかに注目して研究を進めています。そして、分子生物学、遺伝学、生理学、細胞生物学などの手法を用いて、植物独自のストレス適応機構を解明し、植物の巧みな生存戦略を理解することを目標としています。
現在、温室効果ガスの排出が原因による地球温暖化が大きな問題となっています。そして、気候変動に起因する自然災害が地球上の様々な場所で起きています。このような気候変動は農作物の生育にも大きな影響を与え、私達が生きていく上で必要な食料が近い将来に不足すると予測されています。そのことから、私たちの研究を通して、農業生産性の向上やバイオマスの増産、地球環境の改善などにも貢献していきたいと考えています。
研究室メンバー
学部3年 7名研究業績
＜学術論文＞
Takahashi N, Umeda M. (2022) Brassinosteroids are required for efficient root tip regeneration in Arabidopsis. Plant Biotechnol. 39, 73-78.
Zhang X, Nomoto M, Garcia-León M, Takahashi N, Kato M, Yura K, Umeda M, Rubio V, Tada Y, Furumoto T, Aoyama T, Tsuge T. (2022) CFI 25 subunit of cleavage factor I is important for maintaining the diversity of 3′ UTR lengths in Arabidopsis thaliana (L.) Heynh. Plant Cell Physiol. 63, 369-383.
Inada N, Takahashi N, Umeda M. (2021) Arabidopsis thaliana subclass I ACTIN DEPOLYMERIZING FACTORs and vegetative ACTIN2/8 are novel regulators of endoreplication. J. Plant Res. 134, 1291-1300.
Takahashi N, Inagaki S, Nishimura K, Sakakibara H, Antoniadi I, Karady M, Ljung K, Umeda M. (2021) Alterations in hormonal signals spatially coordinate distinct responses to DNA double-strand breaks in Arabidopsis roots. Sci. Adv. 7, eabg0993.
Zhang XL, Wu Q, Tao Y, Zhu XF, Takahashi N, Umeda M, Shen RF, Ma JF. (2021) ANAC044 is associated with P reutilization in P deficient Arabidopsis thaliana root cell wall in an ethylene dependent manner. Environ. Exp. Bot. 185, 104386.
Watanabe S, Takahashi N, Kanno Y, Suzuki H, Aoi Y, Takeda-Kamiya N, Toyooka K, Kasahara H, Hayashi KI, Umeda M, Seo M. (2020) The Arabidopsis NRT1/PTR FAMILY protein NPF7.3/NRT1.5 is an indole-3-butyric acid transporter involved in root gravitropism. Proc. Natl. Acad. Sci. USA 117, 31500-31509.
Yoshiyama KO, Aoshima N, Takahashi N, Sakamoto T, Hiruma K, Saijo Y, Hidema J, Umeda M, Kimura S. (2020) SUPPRESSOR OF GAMMA RESPONSE 1 acts as a regulator coordinating crosstalk between DNA damage response and immune response in Arabidopsis thaliana. Plant Mol. Biol. 103, 321-340.
Takahashi N, Ogita N, Takahashi T, Taniguchi S, Tanaka M, Seki M, Umeda M. (2019) A regulatory module controlling stress-induced cell cycle arrest in Arabidopsis. eLife 8, e43944.
Ogita N, Okushima Y, Tokizawa M, Yamamoto YY, Tanaka M, Seki M, Makita Y, Matsui M, Okamoto-Yoshiyama K, Sakamoto T, Kurata T, Hiruma K, Saijo Y, Takahashi N, Umeda M. (2018) Identifying the target genes of SUPPRESSOR OF GAMMA RESPONSE 1, a master transcription factor controlling DNA damage response in Arabidopsis. Plant J. 94, 439-453.
Chen P, Takatsuka H, Takahashi N, Kurata R, Fukao Y, Kobayashi K, Ito M, Umeda M. (2017) Arabidopsis R1R2R3-Myb proteins are essential for inhibiting cell division in response to DNA damage. Nat Commun. 8, 635.
Davis OM, Ogita N, Inagaki S, Takahashi N, Umeda M. (2016) DNA damage inhibits lateral root formation by up-regulating cytokinin biosynthesis genes in Arabidopsis thaliana. Genes Cells 21, 1195-1208.
Weimer AK, Biedermann S, Harashima H, Roodbarkelari F, Takahashi N, Foreman J, Guan Y, Pochon G, Heese M, Van Damme D, Sugimoto K, Koncz C, Doerner P, Umeda M, Schnittger A. (2016) The plant-specific CDKB1-CYCB1 complex mediates homologous recombination repair in Arabidopsis. EMBO J. 35, 2068-2086.
Yi D, Alvim Kamei CL, Cools T, Vanderauwera S, Takahashi N, Okushima Y, Eekhout T, Yoshiyama KO, Larkin J, Van den Daele H, Conklin P, Britt A, Umeda M, De Veylder L. (2014) The Arabidopsis SIAMESE-RELATED cyclin-dependent kinase inhibitors SMR5 and SMR7 regulate the DNA damage checkpoint in response to reactive oxygen species. Plant Cell 26, 296-309.
Takahashi N, Kajihara T, Okamura C, Kim Y, Katagiri Y, Okushima Y, Matsunaga S, Hwang I, Umeda M. (2013) Cytokinins control endocycle onset by promoting the expression of an APC/C activator in Arabidopsis roots. Curr Biol. 23, 1812-1817.
Takahashi N, Kong S, Umeda M. (2013) Dof transcription factors control the expression of the anaphase promoting complex/cyclosome activator CCS52A1. Plant Biotech. 30, 407-410.
Noir S, Bömer M, Takahashi N, Ishida T, Tsui TL, Balbi V, Shanahan H, Sugimoto K, Devoto A. (2013) Jasmonate controls leaf growth by repressing cell proliferation and the onset of endoreduplication while maintaining a potential stand-by mode. Plant Physiol. 161, 1930-1951.
Kuroda H, Yanagawa Y, Takahashi N, Horii Y, Matsui M. (2012) A comprehensive analysis of interaction and localization of Arabidopsis SKP1-like (ASK) and F-box (FBX) proteins. PLoS One 7, e50009.
Breuer C, Morohashi K, Kawamura A, Takahashi N, Ishida T, Umeda M, Grotewold E, Sugimoto K. (2012) Transcriptional repression of the APC/C activator CCS52A1 promotes active termination of cell growth. EMBO J. 31, 4488-4501.
Hamasaki H, Yoshizumi T, Takahashi N, Higuchi M, Kuromori T, Imura Y, Shimada H, Matsui M. (2012) SD3, an Arabidopsis thaliana homolog of TIM21, affects intracellular ATP levels and seedling development. Mol. Plant 5, 461-471.
Kumar S, Yoshizumi T, Hongo H, Yoneda A, Hara H, Hamasaki H, Takahashi N, Nagata N, Shimada H, Matsui M. (2012) Arabidopsis mitochondrial protein TIM50 affects hypocotyl cell elongation through intracellular ATP level. Plant Sci. 183, 212-217.
Cools T, Iantcheva A, Weimer AK, Boens S, Takahashi N, Maes S, Van den Daele H, Van Isterdael G, Schnittger A, De Veylder L. (2011) The Arabidopsis thaliana checkpoint kinase WEE1 protects against premature vascular differentiation during replication stress. Plant Cell 23, 1435-1448.
Shimada H, Obayashi T, Takahashi N, Matsui M, Sakamoto A. (2010) Normalization using ploidy and genomic DNA copy number allows absolute quantification of transcripts, proteins and metabolites in cells. Plant Methods 6, 29.
Takahashi N, Quimbaya M, Schubert V, Lammens T, Vandepoele K, Schubert I, Matsui M, Inzé D, Berx G, De Veylder L. (2010) The MCM-binding protein ETG1 aids sister chromatid cohesion required for postreplicative homologous recombination repair. PLoS Genet. 6, e1000817.
Takahashi N, Lammens T, Boudolf V, Maes S, Yoshizumi T, De Jaeger G, Witters E, Inzé D, De Veylder L. (2008) The DNA replication checkpoint aids survival of plants deficient in the novel replisome factor ETG1. EMBO J. 27, 1840-1851.
Takahashi N, Nakazawa M, Shibata K, Yokota T, Ishikawa A, Suzuki K, Kawashima M, Ichikawa T, Shimada H, Matsui M (2005) shk1-D, a dwarf Arabidopsis mutant caused by activation of the CYP72C1 gene, has altered brassinosteroid levels. Plant J. 42, 13-22.
Takase T, Nakazawa M, Ishikawa A, Kawashima M, Ichikawa T, Takahashi N, Shimada H, Manabe K, Matsui M. (2004) ydk1-D, an auxin-responsive GH3 mutant that is involved in hypocotyl and root elongation. Plant J 37, 471-483.
Takahashi N, Kuroda H, Kuromori T, Hirayama T, Seki M, Shinozaki K, Shimada H, Matsui M. (2004) Expression and interaction analysis of Arabidopsis Skp1-related genes. Plant Cell Physiol 45, 83-91.
Kuroda H, Takahashi N, Shimada H, Seki M, Shinozaki K, Matsui M. (2002) Classification and expression analysis of Arabidopsis F-box-containing protein genes. Plant Cell Physiol 43, 1073-1085.
＜著書・総説＞
Shimotohno A, Aki SS, Takahashi N, Umeda M. (2021) Regulation of the plant cell cycle in response to hormones and the environment. Annu Rev Plant Biol. 72, 273-296.
高橋直紀, 梅田正明（2020）ストレスに応答した植物の細胞周期停止機構. 植物科学最前線（BSJ-review）11, 3-11.
Umeda M, Aki SS, Takahashi N. (2019) Gap 2 phase: making the fundamental decision to divide or not. Curr Opin Plant Biol. 51, 1-6.
高橋直紀, 梅田正明（2016）根端メリステムのサイズ制御機構. 植物科学最前線（BSJ-review）7, 201-209.
高橋直紀, 梅田正明（2014）植物のDNA損傷応答. 生物と科学 54, 123-129.
Takahashi N, Umeda M. (2014) Cytokinins promote onset of endoreplication by controlling cell cycle machinery. Plant Signal. Behav. 9, e29396.